Fig. 3. DLST knockdown decreases oxygen consumption rates and glutamine anaplerosis in DLST-dependent TNBC cells but not in independent ones.

a Quantification of oxygen consumption rates (OCR) in BT-549 and Hs578T cells after DLST knockdown, assessed through Seahorse technology (n = 6). A representative OCR curve for each cell line was included in (Supplementary Fig. 7). b Scheme of ¹²C and ¹³C labeled glutamine contribution to TCA-cycle metabolites. c, d Percentage of ¹³C labeled glutamine-derived cycle metabolites was measured upon DLST knockdown in BT-549 (c) and Hs578T cells (d) (n = 3). Data are presented as mean ± s.e.m, and an unpaired two-tailed t-test was used for statistical analysis in (a, c, d). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.