Fig. 7. DLST depletion induces ROS, apoptosis, and necrosis while slowing the proliferation of human DLST-dependent TNBC cells, which can be rescued by NAC treatment.

a, b Representative images (a) and quantitation (b) of mitoSOX (red, a marker for mitochondrial ROS), ethidium homodimer III (red, a marker for necrosis), active-caspase 3 (blue, a marker for apoptosis), and Ki-67 (blue, a marker for cell proliferation) stainings, which were overlaid either with GFP or DAPI signals of tumor cells in the presence or absence of NAC treatment (n = 3–10 fish per group). Scale bars = 20 μm. Data in (b) presented as mean ± s.e.m. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. Statistical differences were calculated using one-way ANOVA.