Skip to main content
. Author manuscript; available in PMC: 2022 May 15.
Published in final edited form as: Clin Cancer Res. 2021 Sep 13;27(22):6235–6249. doi: 10.1158/1078-0432.CCR-21-0971

Figure 4.

Figure 4

(A) Flow Cytometric analysis of NK cell populations in MOC2 tumors 3 days after start of treatment with 10 Gy RT, anti-CD25, anti-CD137, compared to RT alone.; *p≤0.1, **p≤0.05; n=5. (B) FlowSOM clustering of raw flow cytometry of NK cell populations in LY2 tumors treated with 10 Gy RT and either isotype antibody or anti-CD25 antibody. Tumors were taken 8 days after RT; *p≤0.1, **p≤0.05; n=5. (C) Flow Cytometry of NK cells in blood of LY2 tumor-bearing BALB/c mice taken 8 days after 10 Gy RT; *p≤0.1, **p≤0.05; n=5. (D) FlowSOM clustering of raw flow cytometry of NK cell populations in MOC2 tumors treated with 10 Gy RT and anti-CD137 antibody with and without anti-CD25 antibody. Tumors were taken 13 days after RT; *p≤0.05; n=5. (E) Flow cytometry of NK cells in blood of MOC2-bearing C57BL/6 mice taken 13 days after 10 gy RT; *p≤0.05; n=5. (F) Tumor volumes of MOC2 tumors from DEREG mice treated with 10 Gy RT, anti-CD137, and diphtheria toxin (DT) (black line) or treated with RT, anti-CD25, anti-CD137, and diphtheria toxin (DT) (green line); n=7. (G) Flow cytometry of NK cell populations in blood from WT C57BL/6 mice treated with 10 Gy RT, anti-CD25, and anti-CD137 and from DEREG mice treated with 10 Gy RT, anti-CD25, anti-CD137, and diphtheria toxin (DT); *p≤0.05; n=5. (H) Heatmap depicting the 14 populations of NK cells identified by FlowSOM, and the relative expression of markers of those populations in WT C57BL/6 mice treated with RT, anti-CD25, and anti-CD137 and in DEREG mice treated with RT, anti-CD137, and diphtheria toxin (DT) or treated with 10 Gy RT, anti-CD25, anti-CD137, and diphtheria toxin (DT); n=5. (I) Frequency of populations identified by FlowSOM in C57BL/6 mice treated with 10 Gy RT, anti-CD25, and anti-CD137 and in DEREG mice treated with 10 Gy RT, anti-CD137, and diphtheria toxin (DT) or treated with RT, anti-CD25, anti-CD137, and diphtheria toxin (DT); n=5.