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. 2021 Nov 16;12:6610. doi: 10.1038/s41467-021-26762-2

Fig. 6. SARS-CoV-2 infection of human pediatric and adult gastric organoids.

Fig. 6

a Immunofluorescence panels for undifferentiated pediatric RP-GOs, showing NP CoV in red and somatostatin (SST) in cyan (left panel), and NP CoV in red and mucin 5AC (MUC5AC) in green (right panel). Scale bars 100 µm. b Cell counting of infected cells, SST, MUC5AC positive and co-positive cells per organoid of pediatric origin. Mean ± SEM (SST n = 11 organoids; MUC5AC n = 16 organoids). c Graphs of SARS-CoV-2 replication in pediatric and adult differentiated RP-GOs. Culture supernatants collected at 0, 24, 48, 72, and 96 h after infection with SARS-CoV-2 (MOI of 10) were titrated for the presence of live virus and viral genome copies by the FFA and qRRT-PCR, respectively. The dotted lines indicate the lower limit of detection. Black circles indicate single data points. Mean ± SD (n = 5 experimental replicates).