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Tat-stimulated transcription from HIV-1 LTR. In vitro transcription reactions were performed with the purified PICs, and the transcripts were labeled with [α-32P]UTP. The runoff transcripts are 168 nt, as indicated. (A) Tat stimulated transcription from the wild-type HIV-1 LTR. (B) Tat was not able to activate transcription from the TAR mutant (TM26) HIV-1 LTR. A comparison of the transcription activities of wild-type HIV-1 LTR (lanes 1 to 3) and TM26 (lanes 4 to 6) is shown.
