Figure 2.
Cultivation of TM7 species from periodontitis. (A) 16S rRNA profiling of 3 patients that were sampled for the cultivation study. (B) Neighbor-joining tree for TM7 using full-length 16S rRNA sequences using MEGA X. Isolated TM7 and their host bacteria are highlighted in blue. Host bacteria alone (C) and with TM7 (D) are imaged by phase-contrast microscopy. Black arrows are showing TM7. Scale bars are 5 μm. (E) FISH composite images of coculture containing TM7 (red) and host bacteria (yellow) stained with 16S rRNA specific DNA probes. See also Figure S2. Scale bars are 5 μm.