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. 2021 Nov 16;11:22351. doi: 10.1038/s41598-021-01465-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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ASC expression according to Oncomine and clinical PDAC tissues. (a) Relative expression of ASC in normal/benign pancreas tissue (N) versus PDAC (T) according to six independent profiling datasets (surveyed using Oncomine). (b) Evaluation of ASC expression in human PDAC tissue according to IHC analysis. Data were generated from three representative PDAC tissues expressing various ASC levels and determined using the anti-ASC antibody. Scale bar: 200 μm. (c) IHC intensity score among 20 evaluated patients. (d) Immune-reaction intensity according to the OD and determined using ImageJ (n = 20). Error bars indicate the standard error of the mean based on eight independent experiments. (e–g) Expression of ASC protein in pancreatic tissue distant form the tumor (e), tissue surrounding the PDAC tissue (f), and PDAC tissue (g) according to IHC analysis. Scale bar: 100 μm.