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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Biochim Biophys Acta Gen Subj. 2021 Oct 6;1866(1):130017. doi: 10.1016/j.bbagen.2021.130017

Figure 2.

Figure 2.

Humanin induces autophagy in several cell lines. (A) HEK293 cells stably expressing mRFP-GFP-LC3 were treated with HNG for 24hr. For quantification of either yellow (mRFP+GFP) or red (mRFP only) puncta, 80 cells were analyzed by Image J. (B) B16 melanoma cells were treated with HNG for 24hr. The expression levels of LC3 and p62/SQSTM1 were measured by western blot (n=3). (C-D) SH-SY5Y neuroblastoma cells were treated with HNG for 24hr. The expression levels of LC3, p62/SQSTM1 (n=3), and autophagy-related genes (n=2) were measured by western blot.

*<0.05,***<0.001.