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Humanin increases autophagy in mouse skeletal muscle and improves muscle function in aged mice. (A-B) Autophagy-related protein levels were measured by qRT-PCR (n=5/group). (C) Western blot showing the LC3 expression and quantification of the LC3-II accumulation (n=4/group). (D) The western blot and quantification of K63-mediated ubiquitinated proteins (n=5/group). (E) Rotarod performance test (n=10/group). # <0.1, *<0.05, **<0.01.
