Figure 4.

Cholesterol decreases the number of content-mixing events that result in content loss but has no effect on the frequency of content loss. (A) Target membrane cholesterol decreases the fraction of content-mixing events that result in content loss. The fraction of content-mixing events that result in content loss for 10, 20, 30, and 40 mol% cholesterol is 64/238, 31/157, 29/215, and 29/380, respectively. (B) Increasing target membrane cholesterol has no significant effect on the fraction of vesicles that undergo content loss with no previous mixing event. The number of content-loss events for 10, 20, 30, and 40 mol% cholesterol is 724/5250, 427/2458, 328/2153, and 333/2411, respectively. Points represent the fraction ± bootstrap resampling error.