Figure 5.

IAV antibodies decrease the frequency of content-loss events to membranes containing 10 and 40 mol% cholesterol. Influenza A virions were incubated with various concentrations of monoclonal antibodies. Content-labeled vesicles with 10 and 40 mol% cholesterol were tethered to virions through DNA-lipid hybridization, and the pH was lowered to trigger content mixing and content loss. For both target membrane compositions, the addition of antibodies to IAV decreased the frequency of content-loss events. Each data point represents the frequency of content loss ± bootstrap resampling error for at least 900 vesicles.