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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Biochim Biophys Acta Mol Cell Biol Lipids. 2021 Oct 2;1867(1):159064. doi: 10.1016/j.bbalip.2021.159064

Figure 1.

Figure 1.

Characterization of single-donor VLDL isolated from plasma of three representative healthy donors from groups 1 (light grey), 2 (black) and 3 (dark grey) (low, medium and high TG). Table 1 shows biochemical composition of these VLDL.

(A) SEC profiles and (B) transmission electron micrographs of VLDL groups 1 to 3 (top to bottom). Peaks I and II, which correspond to VLDL subclasses VLDL1 (large buoyant) and VLDL2 (small dense), are indicated (see supplemental Figure S4 for details). Elution profiles of freshly isolated single-donor human HDL, LDL and VLDL are shown for reference (top).

(C) Non-denaturing PAGE and (D) SDS PAGE of VLDL groups 1 to 3 (left to right). St – molecular standards. Bands corresponding to the major VLDL apolipoproteins are labeled in panel D.

(E) Heparin-sepharose chromatography profiles of VLDL. Bound and non-bound fractions are labeled. The dotted line indicates the calculated NaCl concentration (100% corresponds to 1.01 M NaCl).