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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Biochim Biophys Acta Mol Cell Biol Lipids. 2021 Oct 2;1867(1):159064. doi: 10.1016/j.bbalip.2021.159064

Figure 5.

Figure 5.

VLDL remodeling by the exogenous LpL. (A) Time course of TG lipolysis in group 2 VLDL by bovine LpL (see part 2 Methods for details). The reaction was monitored by quantifying NEFA at indicated time points. (B) Non-denaturing PAGE of VLDL after lipolysis by LpL for indicated times. Band positions corresponding to the particle size of intact VLDL (I), IDL/LDL (II), HDL (III’ and III) and lipid-poor proteins (IV) are indicated. (C) SEC and (D) heparin affinity profiles of VLDL hydrolyzed for indicated times. Peaks I-IV are indicated. The dotted line in D represents the calculated NaCl concentration (100% corresponds to 1.01 M NaCl).