Table 2.

Biochemical composition of nonbound and bound fractions isolated from the same VLDL batch by heparin-sepharose affinity chromatography. Native VLDL (before chromatography) is shown for comparison. Representative data for a single donor from group 2 are shown; the trends are reproducible for all donors within the same group. The corresponding chromatographic profile for group 2 VLDL is shown in Figure 1E (middle panel). TG - triacylglycerol; PL – phospholipid (total); Ch - cholesteryl (total); FC - unesterified (free) cholesterol; CE – cholesterol ester. NEFA values (in nM/mg of apoB), which change over time as shown in Figure 3A below, are provided 6 hours after the isolation of bound VLDL when NEFA values level off. The lipids were quantified by enzymatic assays as described in Methods. The proteins were quantified by ELISA. The values, shown as % of total lipid weight or total protein weight, represent the mean of three independent experiments, with the accuracy of ± 2%. Although LpL has not been quantified, the dot blot data (Figure 4D) show LpL in bound and in native VLDL but not in the nonbound VLDL (indicated by + or −).

	Native	Nonbound	Bound
Lipid, %
TG	40.0 ± 1.2	57.0 ± 1.6 ***	8.3 ± 1.2 ***
PL	27.2 ± 1.8	23.8 ± 1.9	28.5 ± 1.4
Ch	16.4 ± 1.2	10.1 ± 1.1 ***	33.6 ± 1.8 ***
FC	5.1 ± 1.1	3.4 ± 1.1 **	11.8 ± 1.9 ***
CE	11.3 ± 1.4	5.7 ± 1.4 ***	17.8 ± 1.9 ***
NEFA, nM/ mg apoB	65 ± 7.1	52 ± 17	170 ± 15 ***
Protein, %
apoB	45 ± 1.4	42 ± 1.1	35 ± 1.7 **
apoE	22 ± 1.9	7 ± 1.5 ***	39 ± 1.4 ***
apoC-III	21 ± 1.2	49 ± 1.5***	11 ± 1.9 ***
apoC-II	12 ± 1.4	2 ± 1.4 ***	15 ± 1.7
LpL	+	−	+

The results are shown as the mean ±SEM of three independent measurements; p≤0.1 (**), p≤0.05