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. 2021 Nov 16;11:22317. doi: 10.1038/s41598-021-01739-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cell proliferation and ALP activity of Saos-2 on different porous structures. Saos-2 cells were seeded at a density of 2.4 × 10⁴ cells/well and cultured for 14 days. (a) Cell proliferation was measured using CCK-8. (b) The enzyme activity of ALP within cells was measured on days 5 and 14. The trend of ALP activity was similar to that of cell proliferation (mean ± SD).