REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
WNV E60 | Oliphant et al., 2006 | N/A |
DV4 E88 | Sukupolvi-Petty et al., 2013 | N/A |
Anti-mouse Ifnar1 mAb | Leinco | Clone MAR1-5A3; Product No I-1188 |
HRP conjugated anti-mouse IgG (whole molecule) | Sigma | A8924; RRID:AB_258426 |
Bacterial and virus strains | ||
Dengue DKE-121 | Isolated from patient (this study) | N/A |
DENV-1 Western Pacific | Durbin et al., 2006 | N/A |
DENV-2 NGC | Sukupolvi-Petty et al., 2010 | N/A |
DENV-2 D2S20 | Makhluf et al., 2013 | N/A |
DENV-3 16652 | Brien et al., 2010 | N/A |
DENV-4 H-241 | World Reference Center for Emerging Viruses and Arboviruses at UTMB | N/A |
DENV-4 TVP-376 | Sukupolvi-Petty et al., 2013 | N/A |
DENV-4 P75-514 | World Reference Center for Emerging Viruses and Arboviruses at UTMB | N/A |
DENV-4 Dominica/81 | World Reference Center for Emerging Viruses and Arboviruses at UTMB | N/A |
Deposited data | ||
DKE-121 whole genome sequence | This paper | accession number: PRJNA729800 |
Experimental models: Cell lines | ||
Vero CCL81 | ATCC | CCL-81; RRID:CVCL_0059 |
Vero-furin | Mukherjee et al., 2016 | N/A |
Raji-DCSIGNR | Davis et al., 2006 | N/A |
HEK-293T/17 | ATCC | CRL-11268; RRID:CVCL_1926 |
C6/36 | ATCC | CRL-1660; RRID:CVCL_Z230 |
Experimental models: Organisms/strains | ||
C57Bl/6J | Jackson Laboratory | 000664; RRID:IMSR_JAX:000664 |
AG129 | Johnson et al., 1999, Marshall BioResources | AG129 |
hSTAT2-KI | Gorman et al., 2018; Jackson Laboratory | 031630; N/A |
Macaca mulatta | NIHAC, Poolesville MD | N/A |
Oligonucleotides | ||
DENV-2 D2S20 qPCR Primer 1: 5'-ACA GTC AAC CCA ATC GTA ACA-3'; Primer 2: 5'-TTC AAT TGT CCC GGC TCT AC-3'; Probe: /56-FAM/AG GTT CTG C/ZEN/T TCT ATG TTG ACT GGG C/3IABkFQ/ | This paper | N/A |
DENV-4 gII (TVP-376) qPCR: Primer 1: 5'-ACC AAC AGT GTA ACC AAC ATA GA-3'; Primer 2: 5'-TCC TGA ACC AAT GGA GTG TTA AT-3'; Probe: /56-FAM/AG TGA TAG G/ZEN/T GTT GGA AAC AGC GC/3IABkFQ/ | This paper | N/A |
DKE-121 qPCR Primer 1: 5'-GGC AAG CAT AGA GCT GGA A-3'; Primer 2: 5'-TTC TAA ACC AGT GGA GGG TAA TG-3'; Probe: /56-FAM/AC ATT ACC A/ZEN/T TGG AAC AGG AGA TGG GAC /3IABkFQ/ | This paper | N/A |
Software and algorithms | ||
Clustal Omega | Sievers et al., 2011 | N/A |
FigTree | Andrew Rambaut | http://tree.bio.ed.ac.uk/software/figtree/ |
BioSpot | Cellular Technology Limited | N/A |
Python SciPy | Virtanen et al., 2020 | N/A |
Ident and Sim | Stothard, 2000 | |
Aline | Bond and Schüttelkopf, 2009 | N/A |
PyMOL | Schrödinger | https://pymol.org/2/ |