(A) Atomic model showing Pierce1 and Pierce2 spanning the microtubule wall (shown in transparent surface representation) and linking the external ODA-DC to the filamentous MIP CFAP53.
(B and C) Interaction of the Pierce1 (B) and Pierce2 (C) N termini with CFAP53.
(D) Superposition of Pierce1 and Pierce2. A conserved central region interacts with the CCDC114/151 coiled coil. The sequence alignment of this conserved region is shown beneath.
(E) Expanded view showing a potential hydrogen bond between two neighboring CFAP53 molecules involving R158 and Q495. Mutation of R158 to glycine has been implicated casually in dextrocardia (Noël et al., 2016).
See also Video S1.