KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-myosin heavy chain A4.1025, unconjugated | Developmental Studies Hybridoma Bank | DSHB Cat #A4.1025; RRID:AB_528356 |
| Sheep anti-Digoxigenin Fab fragments antibody, alkaline phosphatase conjugated | Roche | Cat #11093274910; RRID:AB_514497 |
| Rabbit anti-acetylated tubulin D20G3, unconjugated | Cell Signaling Technology | Cat #5335; RRID:AB_10544694 |
| Mouse anti-γ-tubulin GTU-88, unconjugated | Sigma | Cat #T6557; RRID:AB_477584 |
| Rabbit anti-Pierce1, polyclonal, unconjugated | This study | N/A |
| Goat anti-rabbit, polyclonal, HRP conjugated | Agilent | Cat #P0448; RRID:AB_2617138 |
| Biological samples | ||
| Bovine trachea | Adam’s Farm (Athol, MA) | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| 1,4-Dithiothreitol (DTT) | Roche | Cat #DTT-RO |
| 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) | Sigma | Cat #C3023-5G |
| 4′,6-diamidino-2-phenylindole (DAPI) | Invitrogen | Cat #D1306 |
| Adenosine 5′-triphosphate (ATP) | Sigma | Cat #A9187 |
| cOmplete Protease Inhibitor Cocktail | Roche | Cat #CO-RO |
| Dulbecco’s Modified Eagle Medium (DMEM), high glucose with GlutaMAX | GIBCO | Cat #10569010 |
| Ethylenediaminetetraacetic acid (EDTA) | Oakwood Chemical | Cat #238173 |
| Fetal Bovine Serum (FBS) | GIBCO | Cat #10500064 |
| FluoSpheres Sulfate Microsphere, 0.2 μm | Invitrogen | Cat #F8848 |
| Haematoxylin solution Gill No. 3 | Sigma-Aldrich | Cat #GHS332 |
| MEM, HEPES, no glutamine | GIBCO | Cat #11544456 |
| Nystatin, anti-fungal agent | GIBCO | Cat #11548886 |
| Phusion High-Fidelity DNA polymerase | NEB | Cat #M0530S |
| Penicillin/streptomycin | GIBCO | Cat #11528876 |
| ProteaseArrest protease inhibitor cocktail | G Bioscience | Cat #786-108 |
| NBT/BCIP solution | Roche | Cat #11681460001 |
| NP-40 detergent | Thermo Fisher Scientific | Cat #85124 |
| Recombinant mouse PIERCE1 | This study | N/A |
| S. pyogenes Cas9 protein | Toolgen | Cat #TGEN_CP1 |
| Sarkosyl | IBI Scientific | Cat #IB07080 |
| Sodium citrate | BDH Chemicals Ltd | Cat #10242 |
| Sodium Hydroxide | Fisher Scientific | Cat #S/4920/60 |
| SulfoLink coupling resin | Thermo Fisher Scientific | Cat #20401 |
| Trizol | Ambion Life Technologies | Cat #15596018 |
| Trypsin, sequencing-grade | Promega | Cat #V5111 |
| Uranyl formate | Electron Microscopy Sciences | Cat # 22451 |
| Critical commercial assays | ||
| Agilent Technologies qPCR Brilliant II SYBR Master Mix | Agilent Technologies | Cat #600828 |
| DIG RNA labeling mix | Roche | Cat #11277073910 |
| High-Capacity cDNA Reverse Transcription Kit | Applied Biosystems | Cat #4368814 |
| MEGAshortscript T7 Transcription Kit | Ambion | Cat #AM1354 |
| Pierce Silver Stain Kit | Thermo Fisher Scientific | Cat #24612 |
| QIAGEN RNeasy mini kit | QIAGEN | Cat #74104 |
| RT-PCR Kit | QIAGEN | Cat #210212 |
| Taq PCR Master Mix Kit | QIAGEN | Cat #201445 |
| TaqMan GTXpress Master Mix | Applied Biosystems | Cat #4401892 |
| T3 transcription kit | Thermo Scientific | Cat #EP0101 |
| T7 transcription kit | Thermo Scientific | Cat #EP0112 |
| Deposited data | ||
| Composite cryo-EM density map of the 48-nm repeat of the doublet microtubule from Bos taurus | This paper | EMDB: EMD-24664 |
| Cryo-EM density map of the ODA core from Bos taurus | This paper | EMDB: EMD-24663 |
| Atomic model of the 48-nm repeat of the doublet microtubule from Bos taurus | This paper | PDB: 7RRO |
| Atomic Model of the Chlamydomonas doublet microtubule | Ma et al., 2019 | PDB: 6U42 |
| Atomic Model of the Chlamydomonas ODA-DC | Walton et al., 2021 | PDB: 7KZO |
| Atomic Model of the Chlamydomonas ODA | Walton et al., 2021 | PDB: 7KZM |
| Subtomogram average of the bovine doublet microtubule | Greenan et al., 2020 | EMDB: EMD-20674 |
| Subtomogram average of the human doublet microtubule | Lin et al., 2014b | EMDB: EMD-5950 |
| Subtomogram average of the zebrafish doublet microtubule | Yamaguchi et al., 2018 | EMDB: EMD-6954 |
| Subtomogram average of the Chlamydomonas doublet microtubule | Owa et al., 2019 | EMDB: EMD-9768 |
| Subtomogram average of the Tetrahymena doublet microtubule | Imhof et al., 2019 | EMDB: EMD-20012 |
| Subtomogram average of the Chinese hamster ovary centriole | Greenan et al., 2018 | EMDB: EMD-7775 |
| Subtomogram average of the bovine basal body | Greenan et al., 2020 | EMDB: EMD-20676 |
| Experimental models: Organisms/strains | ||
| Bacteria: Escherichia coli DH5α Competent Cells | Thermo Fisher Scientific | Cat #18265017 |
| Bacteria: Escherichia coli DH5α Competent Cells | New England Biolabs | Cat #C2987H |
| Zebrafish: AB wild-type strain | Zebrafish International Resource Center (ZIRC) | RRID:ZIRC_ZL1 |
| Zebrafish: pierce1 KO c.178_179ins(29 bp) | This paper | N/A |
| Zebrafish: pierce2 KO c.166_167ins(34bp) | This paper | N/A |
| Zebrafish: pierce1+2 ; pierce2+34 double knockout | This paper | N/A |
| Mouse: Pierce1−/− (1700007K13Riktm2b(EUCOMM)Wtsi) | MGI | RRID:MGI:5756622 |
| Mouse: Pierce2−/− (Ccpg1osem1(IMPC)H) | This paper | N/A |
| Mouse: Pierce1−/− ; Pierce2−/− double knockout | This paper | N/A |
| Oligonucleotides | ||
| A full list is provided in Table S4 | ||
| Recombinant DNA | ||
| Plasmid: pCR II-TOPO | Invitrogen | N/A |
| Plasmid: pBluescript II KS(-) | Stratagene | Cat #212208 |
| Software and algorithms | ||
| BLAST | Altschul et al., 1990 | https://blast.ncbi.nlm.nih.gov/Blast.cgi |
| CCP4 suite | Winn et al., 2011 | https://www.ccp4.ac.uk/ |
| Chimera v1.13.1 or v1.14 | Pettersen et al., 2004 | https://www.cgl.ucsf.edu/chimera/ |
| ChimeraX v1.1 | Pettersen et al., 2021 | https://www.rbvi.ucsf.edu/chimerax/ |
| CHOPCHOP v3 | Montague et al., 2014 | https://chopchop.cbu.uib.no/ |
| Clustal Omega v1.2.2 | Sievers et al., 2011 | http://www.clustal.org/omega/ |
| CMView v1.1.1 | Vehlow et al., 2011 | http://www.bioinformatics.org/cmview/ |
| Coot v0.9-pre or v0.9.4.1 | Emsley and Cowtan, 2004 | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| CTFFIND4 | Rohou and Grigorieff, 2015 | https://grigoriefflab.umassmed.edu/ctffind4 |
| DeepEMhancer | Sánchez-García et al., 2020 | https://github.com/rsanchezgarc/deepEMhancer |
| ESPript 3.0 | Robert and Gouet, 2014 | https://espript.ibcp.fr/ |
| EV-couplings V2 server | Hopf et al., 2019 | https://v2.evcouplings.org/ |
| ImageJ 1.44d | Schneider et al., 2012 | https://imagej.github.io/ |
| MOLREP v11.6 | Vagin and Teplyakov, 2010 | https://www.ccp4.ac.uk/html/molrep.html |
| MotionCor2 | Zheng et al., 2017 | https://msg.ucsf.edu/software |
| phenix.auto_sharpen v1.18.2-3874 | Afonine et al., 2018 | https://phenix-online.org/ |
| phenix.molprobity v1.18.2-3874 | Chen et al., 2010 | https://phenix-online.org/ |
| phenix.real_space_refine v1.18.2-3874 | Afonine et al., 2018 | https://phenix-online.org/ |
| PIVLab plugin for MATLAB | Thielicke and Stamhuis, 2014 | https://www.mathworks.com/matlabcentral/fileexchange/27659-pivlab-particle-image-velocimetry-piv-tool-with-gui |
| Prism v9 | GraphPad | https://www.graphpad.com |
| PSIPRED 4.0 | Jones, 1999 | http://bioinf.cs.ucl.ac.uk/psipred/ |
| RELION-3.1 | Zivanov et al., 2018 | https://www3.mrc-lmb.cam.ac.uk/relion/ |
| SBGrid | Morin et al., 2013 | https://sbgrid.org/ |
| SerialEM 3.7 | Schorb et al., 2019 | https://bio3d.colorado.edu/SerialEM/ |
| SEQUEST | Thermo Fisher Scientific | https://www.thermofisher.com/us/en/home.html |
| SWISS-MODEL | Waterhouse et al., 2018 | https://swissmodel.expasy.org/ |
| TrRosetta | Yang et al., 2020 | https://robetta.bakerlab.org |
| Python 3.8.8 | Python Software Foundation | https://www.python.org/downloads/release/python-388/ |
| Other | ||
| C-flat holy carbon grids (R1.2/1.3, 400 mesh copper) | Electron Microscopy Sciences | Cat #CF413-50 |
| Quantifoil holy carbon grids (R1.2/1.3, 400 mesh gold) | Quantifoil Micro Tools | Cat #Q4100AR1.3 |
| Quantifoil holy carbon grids (R2/2, 400 mesh copper) | Quantifoil Micro Tools | Cat #Q4100CR2 |
| Curated list of tektin sequences | Bastin and Schneider, 2019 | N/A |
| Rat and Mouse Diet No. 3 | Special Diet Services, UK | Cat #RM3 |