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. 2021 Nov 16;11:590. doi: 10.1038/s41398-021-01680-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — 293T cells transiently co-transfected with Flag-tagged WT and mutant SHARPIN and Myc-tagged HOIP were immunoprecipitated using anti-Myc antibody (A) and anti-Flag antibody (B). The binding affinity between SHARPIN variants and HOIP was confirmed through western blot analysis using anti-Myc and anti-Flag antibody, respectively.