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. 2021 Nov 16;11:22349. doi: 10.1038/s41598-021-01072-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Microbial abundances estimated through culture-dependent and culture-independent methods, normalized to gram dry weight (gdw) using moisture content measurements. Error bars show the standard deviation of triplicate culture-based enumerations and the standard deviation of duplicate qPCR amplifications. Measurements for aerobic and anaerobic heterotrophs below 2.5 × 10³ CFU/gdw are below the lower limit of plate count quantification (i.e., 25 colonies per plate) but are shown here for comparison nonetheless. The PLFA analyses were performed in singlicate (duplicate for AB1 and IR1) and only the predicted prokaryotic abundances are reported here for comparison.