FIG. 10.

Effect of genistein on ATF6 activity and protein level. (A) Effect of genistein on hIre1p, ATF6, and yHac1p stimulation of the grp78 promoter. NIH 3T3 cells were transiently transfected with −154CAT and either the empty CMV vector (V) or expression vector for hIre1p, ATF6, or yHac1p as indicated. The cells were either nontreated (−G) or treated with 140 μM genistein (+G). CAT activity in the nontreated cells transfected with the empty CMV vector was set at 1. Relative promoter activities are shown with standard deviations. (B) Effect of genistein on ATF6 protein level. Total cell lysates were prepared from NIH 3T3 cells with no drug treatment or Tg treated for 2 or 10 h, in the presence or absence of genistein (G), as indicated at the top; 30 μg of cell lysate from each sample was applied to an SDS–6% polyacrylamide gel and Western blotted with anti-ATF6 antibody.