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. 2021 Nov 3;11:781720. doi: 10.3389/fonc.2021.781720

Figure 1.

Figure 1

Inhibition of CSC markers and Wnt/β-catenin signaling by 6-gingerol. (A) Western blot analysis of SOX2, OCT4, and NANOG in NCCIT and NTERA-2 cells after treatment with 100 or 200 μM 6-gingerol for 48 h. Expression levels of proteins were estimated by densitometry and normalized to β-actin. Data were obtained in triplicate. (B) Real-time qPCR showing illus-trative expression of SOX2, OCT4, and NANOG genes in embryonic CSCs. The obtained Cp values were normalized to GAPDH mRNA. Controls were set at 100. ***p < 0.001 (ANOVA). # p < 0.001 vs. control. (C) Western blot of Wnt5a, Wnt8A, GSK-3β, β-catenin, and TCF3/TCF7L1 in NCCIT and NTERA-2 cells after treatment with 100 or 200 μM 6-gingerol for 48 h. Expression levels were normalized to β-actin. Experiments were conducted three times for confirmation. (D) Real-time qPCR showing illustrative expression of Wnt5a, Wnt8A, GSK-3β, β-catenin, TCF3, and TCF7 genes in NCCIT and NTERA-2 cells. Cp values were normalized to GAPDH mRNA. Controls were set at 100. ***p < 0.001 (ANOVA). # p < 0.001 vs. control.