Figure 1.

WS and CT iPSCs both efficiently generate homogeneous populations of neural stem cells and neurons

(A) Immunofluorescence microscopy for NESTIN (NSCs marker) and Ki67 (proliferation marker) in NSCs derived from the three control (CT1, CT2, and CT3) and the three WS (WS1, WS2, and WS5) iPSC lines. Scale bars, 100 μm.

(B) HuC/D- and CUX2-immunoreactive cortical neurons at days of in vitro (DIV) differentiation 14 from NSC stage. Scale bars, 100 μm.

(C) Proportion of HuC/D⁺- and CUX2⁺-immunoreactive neurons in cell culture at DIV 14 from NSC stage. Data are presented as mean ± SEM (n = 3 biologically independent differentiations. No statistical difference was observed via one-way ANOVA with Dunnett’s post hoc test).

(D) Immunoblot of wolframin in NSCs and neurons at DIV 21.