Figure 2.

WS neurons display abnormal neurite outgrowth

(A) TUJ1-immunoreactive CT and WS neurons at DIV 21. Scale bars, 100 μm.

(B) Confocal analysis of TUJ1-immunoreactive WS5 neurons at DIV 21. Scale bars, 10 μm.

(C) Quantification of the mean thickness of isolated and bundled neurites in CT and WS neurons.

(D) Quantification of the number of isolated and bundled neurites per mm² over three thickness intervals (0–3, 3–6, and >6 μm) for CT and WS neurons (see material and methods).

(E) Quantification of the mean number of isolated and bundled neurites for CT and WS neurons in the >6 μm interval of thickness. The color code indicating the different cell lines is common to (C), (D), and (E). All the data are presented as mean ± SEM (n = 3 biologically independent differentiations; ^∗∗p < 0.01, ^∗∗∗p < 0.001; Student’s t test).