Figure 8.

Persistence of S. Typhimurium in the spleen and in the gall bladder after intracoelomic inoculation. Five-day-old chicks were inoculated in the coelomic cavity with around 3 × 10⁷ CFU per chick with S. Typhimurium 14028 turboFP650 wild-type (WT; blue diamond), ΔinvA::kan mutant strain (ΔinvA; T3SS-1 defective; green diamond) or the ΔinvA::kan ΔpagN::cm Δrck mutant strain (3Δ; T3SS-1, Rck, PagN defective; yellow diamond). Each week, seven animals were sacrificed and their spleens and gall bladders removed. The kinetics of spleen (a) and gall bladder (b) colonization were monitored each week for a period of 36 days. Results are expressed as number of bacteria (log CFU per gram of organ). The medians are represented by a red dash. A Kruskal–Wallis test was conducted, followed by a Dunn's multiple comparisons test (GraphPad Software). Significance was *p < 0.05.