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. 2021 Oct 7;8(22):2100128. doi: 10.1002/advs.202100128

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© 2021 The Authors. Advanced Science published by Wiley‐VCH GmbH

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Aged breast ECM induces KTB21 cell migration and invasion. A) Cell migration on matrices calculated from time‐lapse images taken at 15 min intervals for 3 h. Top: cell trajectories, bottom: motility. Also see Movies S1 and S2. B) Cell invasion through transwell inserts. Left: invaded cell number against FBS gradient. n = 2 matrices. Right: invaded cell number towards cell‐free matrices in the bottom chamber. n = 2 matrices. Data are presented as the mean ± SD. Statistical tests: two‐tailed student's t‐test.