TABLE 4.
CB-RB inhibits MyoD-induced NIH 3T3 cell differentiation
| Plasmid cotransfecteda | No. of MyoD-positive cells | No. of MHC-positive cells | Inhibition of differentiationb (%) |
|---|---|---|---|
| None | 18 | 18 | 0 |
| pCS2+myc6 | 20 | 19 | 5 |
| pCS2+CB-myc6 | 19 | 16 | 16 |
| pCS2+myc6-RBf1 | 20 | 19 | 5 |
| pCS2+myc6-RBf2 | 21 | 21 | 0 |
| pCS2+CB-myc6-RBf1 | 17 | 6 | 65 |
| pCS2+CB-myc6-RBf2 | 19 | 7 | 63 |
a
Plasmids were cotransfected along with pEMSV-MyoD.
b
Calculated by the formula (number of MyoD-positive cells − number of MHC-positive cells)/number of MyoD-positive cells. Cells were counted from a total of three representative fields.