FIG. 5.

NLS-Gal80GFP is located in the nucleus, while Gal80GFP with the mutant form of the NLS is located in both the cytoplasm and the nucleus. Sc725 (gal80Δ) cells carrying either pGP31 (NLS-Gal80GFP) (A to C) or pGP31m (mNLS-Gal80GFP) (D to F) were grown to late exponential phase in medium containing glucose and then streaked on plates containing galactose-glycerol-lactic acid. Plates were incubated at 30°C for 2 days. Cells were then transferred to liquid medium containing galactose, spotted on slides, and observed through a GFP or DIC filter set.