Table 1.
Primer sequence used for detection of ACE rs4646994, AGT rs699 and AGTR1 rs5186 gene polymorphisms
| Polymorphisms | Sequence (5`- > 3`) | Restriction Enzyme | Product size (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
| ACE rs4646994 |
F: GCCCTGCAGGTGTCTGCAGCATGT R: GGATGGCTCTCCCCGCCTTGTCTC |
– |
I allele: 599 bp D allele: 312 bp |
66 |
| AGT rs699 |
F: CCGTTTGTGCAGGGCCTGGCTCTCT R: CAGGGTGCTGTCCACACTGGACCCC |
Tth111I |
T allele: 165 C allele: 141 + 24 |
68 |
| AGTR1 rs5186 |
F: AGAAGCCTGCACCATGTTTTGAG R: CCTGTTGCTCCTCTAACGATTTA |
DdeI |
A allele: 410 bp C allele: 292 + 118 |
60 |