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. Author manuscript; available in PMC: 2021 Nov 17.
Published in final edited form as: Stem Cell Res. 2021 Oct 3;56:102560. doi: 10.1016/j.scr.2021.102560

Fig. 2.

Fig. 2.

Role of calcium in differentiation and surface marker expression on young and old MSCs. A Immunophenotyping assay was done to study the CD44 surface marker expression after various calcium treatment (0 mm Ca2+, 2 and 5 mM Ca2+ for 3 days). B, shows the histogram showing the expression of the CD44 surface marker expression in varying dose of Ca2+ treatment. C Representative traces showing changes in intracellular Ca2+ levels after various Ca2+ treatment. D represent CD44 expression in aged MSCs in various Ca2+ concentrations. Young MSCs grow in in 2 mM Ca2+ was used as control. E Adipocyte and osteocyte differentiation assay was performed to study the stem cell differentiation potential of aged MSCs after treatment with different doses of Ca2+.