FIG. 2.

Sequences of the C-terminal extension of CRAM in different cell lines. (A) DNA nucleotide and amino acid sequences of the transmembrane and cytoplasmic domains of CRAM in wild-type trypanosomes and cell lines containing truncated CRAM cytoplasmic domain. Dotted lines indicate sequences of 100% homology. Changes of nucleotide sequences in different cell lines are indicated by underlines, and the diagnostic restriction enzyme sites resulting from mutations are indicated by typed-out sequences. (B) Comparison of the amino acid sequences of the transmembrane domain and the cytoplasmic domain of CRAM in the wild-type trypanosome and cell lines CRAM-XCD, CRAM-XTM.CD, and CRAM-XTM. The transmembrane domain is underlined. Sequences derived from the ISG65 gene are indicated by boldface.