FIG. 8.

Subcellular localization of different versions of CRAM in bloodstream-form cell lines. Trypanosomes were first incubated with rabbit-derived anti-CRAM antibody followed by reaction with FITC-conjugated goat anti-rabbit IgG. Then cells were stained with DAPI at a concentration of 0.1 μg/ml for 1 min. The images were analyzed by a confocal microscope. Different colors, arranged in the order blue-green-yellow-red-white, indicate the relative intensity of fluorescence (white, highest intensity; blue, lowest intensity). (a) Confocal image of the CRAM protein in the BS-CRAMH23HB cell line; (b) confocal image of total cellular CRAM protein in the BS-CRAM-40 cell line; (c1 and c2) surface staining of fixed, nonpermeabilized BS-CRAM-40 cells (c1, CRAM image; c2, DAPI staining).