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. 2000 Jul;20(14):5149–5163. doi: 10.1128/mcb.20.14.5149-5163.2000

FIG. 9.

FIG. 9

Immuno-EM localization of the CRAM protein in bloodstream-form CRAM overexpressor and BS-CRAM-40 cell line. (A and B) BS-CRAMH23HB probed with rabbit anti-CRAM (18 nm) and mouse MAb Ali I-218 (12 nm) directed against p67, a lysosomal glycoprotein (22). The label for CRAM is observed at the flagellar pocket and the Golgi in the BS-CRAMH23HB cell line. (C) The BS-CRAM-40 cell line probed with rabbit anti-CRAM (18 nm) and mouse MAb Ali I-218 against p67 (12 nm), showing that a significant amount of the CRAM protein was spread onto the surface in the BS-CRAM-40 cell line. fp, flagellar pocket; f, flagellum; g, Golgi; L, lysosome; n, nucleus; s, surface. Scale bars, 0.5 μm.