TABLE 1.
Comparison of subcellular localization of CRAM in cell lines
| Cell line | Subcellular localization of CRAMa
|
|
|---|---|---|
| Total cell staining | Surface staining | |
| Wild type | FP | (FP) |
| CRAM-0 | FP | (FP) |
| CRAM-2 | FP | (FP) |
| CRAM-4 | FP | (FP) |
| CRAM-8 | ER | UD |
| CRAM-14 | ER | UD |
| CRAM-19 | ER | UD |
| CRAM-29 | ER, FP, F | F, (FP, S) |
| CRAM-40 | ER, FP, F | F, (FP, S) |
| CRAM-XTM | FP | (FP) |
| CRAM-XTM.CD | ER | UD |
| CRAM-XCD | ER | UD |
a
Total cell staining represents the result of staining with fixed and permeabilized trypanosomes; surface staining represents the result of staining with live trypanosomes. FP, flagellar pocket; F, flagellum; ER, endoplasmic reticulum; S, cell surface; UD, under detection level. Parentheses indicate that staining was very weak and/or could be observed only in a subpopulation of trypanosomes on each slide.