Table 2.

Primers for PCR amplification of PDCD1 SNPs

PCR primer pair number	Target SNPs	Upstream primer (5′‐>3′)	Downstream primer (5′‐>3′)	PCR product size (bp)
1	PD1.1 (rs36084323)	AAGCCATTTCACAAACGCCG	CATTCTGTCGGAGCCTCTGG	408
2	PD1.3 (rs11568821)	GCCTGCAGGACTCACATTCT	CTGTGTGATCTGGGGACACC	422
3	PD1.4 (rs6705653) PD1.5 (rs2227981) PD1.9 (rs2227982)	GGTGTCCCCAGATCACACAG	TTCTCCTGAGGAAATGCGCT	478
4	PD1.6 (rs10204525)	ATCCTACGGTCCCAAGGTCA	GCAGTGTGTGGATGTGAGGA	268

PCR, polymerase chain reaction; SNPs, single nucleotide polymorphisms.