Figure 3. N⁶-mA antagonizes SATB1 binding to the chromatin during TSC development.

a. SATB1 binding is inversely correlated with N⁶-mA levels. Left: Western blot of SATB1 expression during cell fate transition at indicated time points. The experiments were repeated independently 3 times with similar results. Right: Number of SATB1 ChIP-seq peaks at indicated days. For blot source data, see Supplementary Figure 1.

b. Aggregation of differential signals of N⁶-mA DIP and SATB1 ChIP centered at differentially increased N⁶-mA peaks (day 5 vs. day 0).

c. Number of SATB1 ChIP-seq peaks in control or Alkbh1 OE cells.

d. Top: Venn diagram showing the intersection between N⁶-mA differentially decreased peaks and SATB1 ChIP-seq differentially increased peaks in Alkbh1 OE vs. control (CT) cells. Bottom: Overlap between the intersecting regions and ssDNA-seq peaks, not to scale. Number of peaks per dataset given in diagram; empirical P value computed versus genome random.

e. Average signal and heat maps of SATB1 ChIP-seq around N⁶-mA peak centers in control or Alkbh1 OE cells.