Figure 2.

The gnt2-1 mutant enhances the punctate pattern of callose deposition under prolonged stress conditions. In situ callose deposition at the root tip was visualized by aniline blue staining and viewed by fluorescence microscopy. (A–X) Five-day-old Col-0, gnt2-1, OE-1, and hex1,2&3 plants were further grown on MS medium (A–D, M–P), MS medium supplemented with 130mm NaCl (E–H, Q–T) or MS medium supplemented with 50μgl⁻¹ TM (I–L, U–X) for 4day, respectively, and stained with 0.005% discolorized aniline blue. The punctate pattern of callose deposition was visualized by using a fluorescent microscope equipped with a DAPI filter set. Notice the strong punctate callose deposition in gnt2-1 plants under prolonged stress conditions (R,V). Scale bar in mock and TM treatment=100μm, scale bar in NaCl treatment=50μm.