Figure 3.

Expression of UPR-related genes is higher in the gnt2-1 mutant than in Col-0 plants under prolonged ER stress. (A) Analysis of GnTII expression in Col-0, gnt2-1, OE-1, and hex1,2&3 plants. (B–I) Relative expression of mRNAs encoding various UPR-related genes in Col-0, gnt2-1, OE-1, and hex1,2&3 plants analyzed by qPCR. Total RNA was extracted from 12-day-old seedlings grown on MS medium with or without TM (20μgl⁻¹). The experiment was performed independently three times (three biological replicates) and normalized with respect to β-TUBULIN expression. Data represent mean±SEM of three independent experiments. Asterisks indicate statistically significant differences (^*p<0.05, ^**p<0.01, and ^***p<0.001) as determined by two-way ANOVA.