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. 2021 Nov 2;12:761064. doi: 10.3389/fpls.2021.761064

Figure 4.

Figure 4

GnTII is involved in dark-induced leaf senescence. (A) Phenotypes of leaves from 4-week-old (0-day) Col-0, gnt2-1, OE-1, and hex1,2&3 plants. Senescence symptoms of detached leaves from the Col-0, gnt2-1, OE-1, and hex1,2&3 plants after 3day of dark treatment. The leaves are arranged from oldest (left) to youngest (right). (B) Chlorophyll contents of Col-0, gnt2-1, OE-1, and hex1,2&3 plants. Chlorophyll content was determined in leaves from 4-week-old (0-day) plants and leaves following a 3-day dark treatment. (C) Relative expression of the senescence-related genes EIN3, ORE1, NYE1, and ACS2 in leaves from the Col-0, gnt2-1, OE-1, and hex1,2&3 plants analyzed by qPCR. Total RNA was extracted from 4-week-old plants before and after 3day of dark treatment. The experiment was performed independently three times (three biological replicates) and normalized with respect to β-TUBULIN expression. Data represent mean±SEM of three independent experiments. Asterisks indicate statistically significant differences (*p<0.05, **p<0.01, and ***p<0.001) as determined by two-way ANOVA.