Figure 5.

Cytokinin signaling is impaired in the gnt2-1 mutant. (A–F) Relative expression of the cytokinin-related genes AHK2 (A), AHP1 (B), AHP2 (C), ARR2 (D), ARR10 (E), and ARR12 (F) in leaves from Col-0, gnt2-1, OE-1, and hex1,2&3 plants analyzed by qPCR. Total RNA was extracted from 4-week-old plants before and after 3day of dark treatment. The experiment was performed independently three times (three biological replicates) and normalized with respect to β-TUBULIN expression. Data represent mean±SEM of three independent experiments. Asterisks indicate statistically significant differences (^*p<0.05, ^**p<0.01, and ^***p<0.001) as determined by two-way ANOVA.