Figure 6.

The gnt2-1 mutant exhibits partial cytokinin insensitivity. (A,B) Representative photographs of Arabidopsis seedlings grown vertically on tissue culture plates containing solidified MS medium (A) or MS medium supplemented with 40nm BAP (B), taken 12day after plating. The grid on the square plate is 18×18mm. (C) Relative root length of Col-0, gnt2-1, OE-1, OE-2, OE-3, and hex1,2&3 plants grown in the absence or presence of BAP. (D–I). Expression of cytokinin-related genes in Col-0, gnt2-1, OE-1, and hex1,2&3 plants. The relative expression of mRNAs encoding the cytokinin-related genes was analyzed by qPCR. The experiment was performed independently three times (three biological replicates) and normalized with respect to β-TUBULIN expression. Data represent mean±SEM of three independent experiments. Asterisks indicate statistically significant differences (^*p<0.05, ^**p<0.01, and ^***p<0.001) as determined by two-way ANOVA.