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. 2021 Nov 2;12:761064. doi: 10.3389/fpls.2021.761064

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Copyright © 2021 Yoo, Ko, Vu, Lee, Yoon, Pham, Kim, Lim, Kang, Hong and Lee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The stability and subcellular localization of PIN2-GFP are changed in gnt2-1 under stress conditions. (A,B) Arabidopsis seedlings grown on tissue culture plates containing MS medium supplemented with 60mm NaCl for 7day were used. (A) The expression of PIN2:PIN2-GFP in Col-0 and gnt2-1 background. The PIN2:PIN2–GFP localization in the root apical tissues of Col-0 and gnt2-1 was visualized under a confocal laser scanning microscope (FV1000; Olympus). Scale bar=40μm. (B) Immunoblot analysis of PIN:PIN2-GFP in Col-0 and gnt2-1 mutant background. The blots were probed with a monoclonal GFP antibody. Ponceau S staining shows equal protein loading.