REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-SARS-CoV Spike Antibody pAb	Sino Biological Inc.	Cat #40150-V08B1
Anti-SARS-CoV-2 Spike antibody pAb	Sino Biological Inc.	Cat #40591-T62
Anti-SARS-CoV Spike antibody (1A9)	GeneTex	Cat #GTX632604
Anti-SARS-CoV nucleocapsid protein polyclonal rabbit antibody	Prospec	Cat # ANT-180
Anti-hPIV2 NP (20A) mAb	Tsurudome et al. (1989)	N/A
Anti-hPIV2 P (211A) mAb	Tsurudome et al. (1989)	N/A
Anti-hPIV2 P/V (315-1) mAb	Nishio et al. (1997)	N/A
Anti-GAPDH, Monoclonal antibody Peroxidase Conjugated	FUJIFILM Wako Pure chemical	Cat #015-25473
Anti-rabbit IgG, HRP-linked Antibody	Cell Signaling Technology	Cat #7074
Anti-mouse IgG, HRP-linked Antibody	Cell Signaling Technology	Cat #7076
Goat Anti-Mouse IgG H&L Alexa Flour488	Abcam	Cat #Ab150113
Goat Anti-Rabbit IgG H&L Alexa Flour568	Abcam	Cat #Ab175471
HRP-Goat anti mouse IgG	BioLegend	Cat #405306
anti-Mouse-IgA HRP	Southern Biotech	Cat #1040-05
Anti-mouse CD28 (37.51)	BioLegend	Cat #102115
PerCP/Cyanine5.5 anti-mouse CD4 Antibody (GK1.5)	BioLegend	Cat #100434
PerCP/Cyanine5.5 anti-mouse CD8a Antibody (53-6.7)	BioLegend	Cat #100734
PE anti-mouse IFN-γ Antibody (XMG1.2)	BioLegend	Cat #505808
Biotinylated anti-rabbit IgG antibody	Vector Laboratory	Cat #BA-1000-1.5
Bacterial and virus strains
BC-PIV	Ohtsuka et al. (2019)	N/A
BC-PIV/S1	This paper	N/A
BC-PIV/S1M	This paper	N/A
BC-PIV/S-2P	This paper	N/A
BC-PIV/S-2PM	This paper	N/A
SARS-CoV-2 (UT-NCGM02)	Imai et al. (2020)	N/A
SARS-CoV-2 Wuhan strain (NC002)	This paper	N/A
SARS-CoV-2 β strain (B.1.351; MD-HP0154)	This paper	N/A
SARS-CoV-2 δ strain (B.1.617.2; UW-5250)	This paper	N/A
Biological samples
Guinea Pig erythrocyte	Japan Bio Serum	Cat #35-0012
Chemicals, peptides, and recombinant proteins
X-treme GENE HP	Roche	Cat #06366244001
DAPI	Nacalai Tesque	Cat #19174-31
Brefeldin A	Biolegend	Cat #420601
SARS-CoV-2 Spike Glycoprotein	GenScript	Cat #RP30020
SARS-CoV-2 Spike S1 Protein	Sino Biological Inc.	Cat #40591-V02H
SARS-CoV-2 Spike Protein RBD	Sino Biological Inc.	Cat #40592-V02H
Human ACE2 Protein	AcroBiosystems	Cat #AC2-H5257
Dako REAL EnVision Detection System	Dako	Cat #K5007
Critical commercial assays
SARS-CoV-2 Surrogate Virus Neutralization Test Kit	GenScript	Cat #L00847
eBioscience™ Intracellular Fixation & Permeabilization Buffer Set	Thermo Fisher Scientific	Cat #88-8824-00
Experimental models: Cell lines
Monkey: Vero	Riken BRC CELL BANK	Cat #RCB0001
Monkey: Vero/BC-F	Ohtsuka et al. (2014)	N/A
Monkey: VeroE6/TMPRSS2	JCRB	Cat #1819
Experimental models: Organisms/strains
Mouse: BALB/c	Japan CLEA	N/A
Hamster: Syrian	Japan SLC	N/A
Oligonucleotides
pcDNA/SARS-CoV-2 S Fw: CAGTGTGGTGGAATTCATGTTTGTGTTCCTGGTGCTG	This paper	N/A
pcDNA/SARS-CoV-2 S Rv : GATATCTGCAGAATTCTCAGGTGTAGTGCAGTTTCAC	This paper	N/A
Vec/SARS-CoV-2 Fw 1: TCTCTATCTGCGGCCGCATATATGTTTGTGTTCCTGGTGCTG	This paper	N/A
Vec/SARS-CoV-2 Fw 2: TCTCTATCTGCGGCCGCAATGTTTGTGTTCCTGGTGCTG	This paper	N/A
Vec/SARS-CoV-2 Rv: AATAGAGATGCGGCCGCCTAACCCGTCCGGGCCTATG	This paper	N/A
SARS-CoV-2 S1/S2 Fw: GAGGGCAAGGTCTGTGGCAAGCCAGAGCATC	This paper	N/A
SARS-CoV-2 S1/S2 Rv: TTGCCACAGACCTTGCCCTCCTTGGGCTGTTG	This paper	N/A
SARS-CoV-2 S2P Fw: ACTGGACCCCCCCGAGGCTGAGGTCCAGATTGAC	This paper	N/A
SARS-CoV-2 S2P Rv: CAGCCTCGGGGGGGTCCAGTCTGCTCAGGATGTC	This paper	N/A
PIV2 F/M Fw: ATAGCATTAATACTATCAGTG	This paper	N/A
SARS-CoV-2 S1/PIV2 F /M Rv: CACTGATAGTATTAATGCTATTGGGCTGTTGGTCTGGGTCTG	This paper	N/A
SARS-CoV-2 S/PIV2 F/M Rv: ACTGATAGTATTAATGCTATTGGCCACTTGATGTATTGTTC	This paper	N/A
PIV2 IG Fw: CTCTCATAATTTAAGAAAAAATC	This paper	N/A
SARS-CoV-2 S1/PIV2 IG Rv: TTTTTCTTAAATTATGAGAGTTATGGGCTGTTGGTCTGGGTC	This paper	N/A
SARS-CoV-2 S/PIV2 IG Rv: TTTTTCTTAAATTATGAGAGTCAGGTGTAGTGCAGTTTCAC	This paper	N/A
Recombinant DNA
pCMV3-2019-nCoV-S1-long	Sino Biological Inc.	Cat #VG40591-UT
pCMV3-2019-nCoV-S2-long	Sino Biological Inc.	Cat #VG40590-UT
pcDNA3.1	Invitrogen	Cat #V79020
pcDNA-SARS-CoV-2 S	This paper	N/A
pcDNA-SARS-CoV-2 S-2P	This paper	N/A
phPIV2ΔF (BC-PIV)	Ohtsuka et al. (2014)	N/A
phPIV2ΔF-SARS-CoV-2 S1 (BC-PIV/S1)	This paper	N/A
phPIV2ΔF-SARS-CoV-2 S1M (BC-PIV/S1M)	This paper	N/A
phPIV2ΔF-SARS-CoV-2 S-2P (BC-PIV/S-2P)	This paper	N/A
phPIV2ΔF-SARS-CoV-2 S-2PM (BC-PIV/S-2PM)	This paper	N/A
pCAGGS-PIV2 NP	Ohtsuka et al. (2014)	N/A
pCAGGS-PIV2 P	Ohtsuka et al. (2014)	N/A
pCAGGS-PIV2 L	Ohtsuka et al. (2014)	N/A
SRα-T7	Ohtsuka et al. (2014)	N/A
Software and algorithms
FlowJo (version 7.2.5)	BD Biosciences	N/A
R (version 4.0.2)	The R Foundation	https://www.r-project.org/