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. 2021 Sep 12;22(21):3099–3106. doi: 10.1002/cbic.202100434

Figure 2.

Figure 2

(a) Chimeric xrRNAΔP4−L4−siRNA construct. The stem‐loop P2‐L2 of the subclass 1a xrRNA shown in Figure 1 was exchanged with an siRNA (blue). Antisense and sense sequences, strand 1 (S1) and strand 2 (S2), stems P1 and P3, loop L3, pseudoknots Pk1 and Pk2, and a base triple (BT) are labelled. Base‐pairing interactions are indicated with dashed lines. An adapter is yellow. (b) Non‐denaturing polyacrylamide gel mobilities of xrRNAΔP4−L4−siRNA constructs. Reference bands in the ladder are single‐stranded RNA. (c) Non‐denaturing polyacrylamide gel mobilities of xrRNAΔP4−L4−siRNA constructs with a two‐fold molar excess of S1 to S2 following incubation with Xrn1 at 37 °C for 20 min. The excess S1 is labelled as a single‐stranded RNA (ssRNA). (d) Non‐denaturing polyacrylamide gel mobilities of a control comprising DENVΔP4−L4−siREN S1 and siREN AS, which lacks the xrRNA 3WJ and pseudoknots, and a control siRNA (siREN) following incubation with Xrn1 at 37 °C for 20 min.