FIG. 3.

Endogenous MCF-7 HEF1 is cleaved by a caspase 3-like activity. (A) MCF-7 cells treated with TNF-α (100 ng/ml). (B) MCF-7 cells treated with TNF-α (100 ng/ml) in the presence (+) or absence (--) of the caspase 3 inhibitory peptide z-DEVD-fmk at a final concentration of 25 μM. (C) WEHI 231 cells were treated with α-IgM antibodies (1 μg/ml) and extracted at the indicated times. Total proteins were extracted at the indicated time points, and Western blots were probed with the antibodies (α) indicated on the right.