FIG. 5.

A 28-kDa caspase cleavage product is generated from both HEF1 and p130^Cas. (A) MCF-7 cells were treated with TNF-α (100 ng/ml), and total protein lysates were probed with anti-HEF1-p130^Cas antibody (α-HEF1/p130^Cas). (B) MCF-7 cells were treated with TNF-α (100 ng/ml) in the presence (+) or absence (--) of the caspase 3 inhibitory peptide z-DEVD-fmk at a final concentration of 25 μM. Western blots were probed with α-HEF1/p130^Cas. (C) HeLa cells were transfected with the empty vector (V), HEF1 (H), HEF1 carboxy-terminally tagged with the Myc epitope (H-M), or p130^Cas (p130). Western blots were probed with α-HEF1/p130^Cas. (D) Diagram of the HEF1 caspase cleavage sites and the pieces that are generated.