Figure 5.

Inhibitory effect of β‐d‐xylo‐configured cyclophellitol 1 and cyclophellitol aziridine 2 on β‐glucosidases in intact HEK293T cells expressing GBA, GBA2 and GBA3. (A) Representative gel images of cABPP where cells were treated for 24 h with indicated inhibitor. Lysates were then prepared and labeled with fluorescent ABP 10. Fluorescently labeled proteins were visualized after SDS‐PAGE (1 set from n=3 replicates). (B) IC₅₀ curves determined by cABPP labeling results. (C) Apparent IC₅₀ values towards β‐glucosidases in intact HEK293T cells producing GBA, GBA2 and GBA3 were determined by the fluorescence quantification based on cABPP SDS‐PAGE results.