TABLE 2.
The major EIC peaks analyzed in this study is listed in chromatographic elution order
| Unique ID | Retention time (min) | m/z | Composition | Glycan class | Fucose | Sialic acid |
|---|---|---|---|---|---|---|
| 1 | 7.9 | 1,073.1 | not glycan | Not applicable | ||
| 2 | 8.9 | 1,145.1 | not determined | Not determined | ||
| 3 | 11.6 | 1,003.4 | H9N2 | Oligo mannose | – | – |
| 4 | 11.8 | 922.3 | H8N2 | Oligo mannose | – | – |
| 5 | 12.4 | 1,075.3 | Not determined | Not determined | ||
| 6 | 12.5 | 1,073.1 | not glycan | Not applicable | – | – |
| 7 | 14.6 | 1,356.6 | H5N2 | Oligo mannose | – | – |
| 8 | 18.9 | 1,269.0 | H7N6F1S4 | complex | yes | Yes |
| 9 | 20.1 | 1,101.0 | H5N4F1S1 | Complex | Yes | Yes |
| 10 | 20.4 | 1,318.9 | H5N4F2S2 | Complex | Yes | Yes |
| 11 | 22 | 1,202.9 | H5N5F1S1 | Complex | Yes | Yes |
| 12 | 22.1 | 1,246.3 | H5N4F1S2 | Complex | Yes | Yes |
| 13 | 23 | 1,150.4 | H4N6F2 | Complex | Yes | |
| 14 | 23.3 | 1,101.0 | H5N4F1S1 | Complex | Yes | Yes |
| 15 | 23.6 | 1,028.5 | H5N4F2 | Complex | Yes | |
| 16 | 23.8 | 1,121.3 | H4N5F1S1 | Complex | Yes | Yes |
| 17 | 24.4 | 1,178.5 | H3N2F1 | Paucimannose | Yes | |
| 18 | 24.7 | 1,222.8 | H4N6F1S1 | Complex | Yes | Yes |
| 19 | 25.1 | 1,150.4 | H4N6F2 | Complex | Yes | |
| 20 | 26.4 | 1,202.9 | H5N5F1S1* | Complex | Yes | Yes |
| 21 | 26.5 | 1,101.0 | H5N4F1S1 | Hybrid | Yes | Yes |
| 22 | 27 | 1,075.3 | Not determined | Not determined | ||
| 23 | 27.1 | 1,048.6 | H4N5F2 | Complex | Yes | |
| 24 | 29.8 | 1,381.3 | H3N2F1 | Truncated | Yes | |
| 25 | 30 | 1,192.4 | H4N5F2S1 | Complex | Yes | Yes |
| 26 | 30.1 | 975.8 | H3N5F1* | Complex | Yes | |
| 27 | 30.3 | 1,246.3 | H5N4F1S2 | Complex | Yes | Yes |
| 28 | 30.3 | 894.5 | H3N5F1* | Complex | Yes | |
| 29 | 30.6 | 1,121.3 | H4N5F1S1 | Complex | Yes | Yes |
| 30 | 31.3 | 1,150.4 | H4N6F2 | Complex | yes | |
| 31 | 31.8 | 1,222.8 | H4N6F1S1* | Complex | yes | Yes |
Some of the compositions have been separated on the C18 column into several peaks eluting at different time points. Compositions labeled with “*" also carry bisecting GlcNAc structure confirmed with MS/MS. Composition key: H (hexose), N (N‐acetylhexosamine), F (fucose), S (sialic acid)