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. 2021 Sep 12;22(21):3049–3059. doi: 10.1002/cbic.202100287

Figure 2.

Figure 2

PHF6 fibrillizes spontaneously by incubation at room temperature. The assay was performed using 50 μM PHF6 in NaPi buffer with 10 μM ThT (gray column). NaPi and 10 μM ThT without addition of PHF6 was used as control (red column). Peptides MMD3 or MMD3rev were added in concentrations of 500 μM to 50 μM PHF6 samples (green column and blue column, respectively). Fluorescence was measured at 490 nm in relative units (mean +/− standard deviations of results, three replicates per run). Samples treated with D‐peptides do not show a decreasing ThT signal, indicating no inhibition of PHF6 aggregation. (B) PHF6* fibrillization was performed by incubating 100 μM PHF6* in NaPi buffer with 10 μM ThT at room temperature (gray column). NaPi and 10 μM ThT without addition of PHF6 was used as control (red column). Peptides MMD3 or MMD3rev were added in concentrations of 1000 μM to 100 μM PHF6* samples (green column and blue column respectively). Fluorescence was measured at 490 nm in relative units (mean +/− standard deviations of results, three replicates per run). Samples treated with D‐peptides show a decreasing ThT signal, indicating an inhibition of PHF6* aggregation.