Table 2.

Analysis of MYCN gene in 4 indicators and the mean of PCR efficiency per method

Methods	Bias(0.001)	Bias(1)	Relative error	Coefficient of variation	Precision	Resolution	−E
CqMAN	0.0000	0.0000	0.0873	10.86%	0.0025	2.0132	1.8300
Cy0	0.0000	0.0000	0.0935	10.42%	0.0027	2.1344	NA
LinRegPCR	0.0008	0.0869	0.4126	11.32%	0.0039	1.5326	1.8690
PCR-Miner	0.0001	0.0054	0.1434	12.39%	0.0057	2.0524	1.9905
5PSM	0.0057	0.2633	2.5536	41.40%	0.0409	7.0584	1.7462
DART	0.0022	0.1723	1.0169	39.86%	0.0308	7.1726	1.9047
FPLM	0.0008	0.0617	0.6724	50.71%	0.0609	7.4493	1.9844
FPK-PCR	0.0007	0.1672	0.5012	29.55%	0.0236	7.8181	2.3011